Sol-gel derived nanostructured cerium oxide film for glucose sensor

Abstract

Sol-gel derived nanostructured cerium oxide $\left(\mathrm{Ce}{\mathrm{O}}_2\right)$ film deposited on gold (Au) electrode has been utilized for physisorption of glucose oxidase (GOx). X-ray diffraction, atomic force microscopy, UV-visible spectroscopy, and electrochemical techniques have been used to characterize sol-gel derived $\mathrm{Ce}{\mathrm{O}}_2∕\mathrm{Au}$ electrode and $\mathrm{G}\mathrm{O}\mathrm{x}∕\mathrm{Ce}{\mathrm{O}}_2∕\mathrm{Au}$ bioelectrode. The response characteristics of the glucose bioelectrode $(\mathrm{G}\mathrm{O}\mathrm{x}∕\mathrm{Ce}{\mathrm{O}}_2∕\mathrm{Au})$ indicate linearity, detection limit and shelf-life as $50–400\mathrm{mg}∕\mathrm{dL}$ , $12.0\mu \mathrm{M}$ , and $12\text{weeks}$ , respectively. The value of apparent Michaelis–Menten constant $\left(K_m\right)$ of $\mathrm{G}\mathrm{O}\mathrm{x}∕\mathrm{Ce}{\mathrm{O}}_2∕\mathrm{Au}$ bioelectrode has been found to be $13.55\mu \mathrm{M}$ .