Development and Use of a Vaccinia Virus Neutralization Assay Based on Flow Cytometric Detection of Green Fluorescent Protein
Open Access
- 1 October 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (19), 10488-10503
- https://doi.org/10.1128/jvi.77.19.10684-10688.2003
Abstract
A rapid and sensitive neutralization assay is required to evaluate alternative smallpox vaccines. Here we describe the development and use of a 96-well plate, semi-automated, flow cytometric assay that uses a recombinant vaccinia virus expressing enhanced green fluorescent protein and which would be applicable to other viruses.Keywords
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