Preparation of Cell Cultures and Vaccinia Virus Stocks
Open Access
- 1 July 1998
- journal article
- unit
- Published by Wiley in Current Protocols in Molecular Biology
- Vol. 43 (1), 16.16.1-16.16.13
- https://doi.org/10.1002/0471142727.mb1616s43
Abstract
This unit describes the maintenance of cell lines used with vaccinia virus, both in monolayer cultures and in suspension. The suspended cell culture is then used in the preparation of vaccinia virus stocks. The preparation of chick embryo fibroblasts (CEF) is also presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Additionally, support protocols are presented for the titration of standard and MVA vaccinia virus stocks.Keywords
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