Rapid Cellular Internalization of Multifunctional Star Polymers Prepared by Atom Transfer Radical Polymerization
- 12 August 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 11 (9), 2199-2203
- https://doi.org/10.1021/bm1006272
Abstract
Poly(ethylene glycol) (PEG) star polymers containing GRGDS (Gly-Arg-Gly-Asp-Ser) peptide sequences on the star periphery were synthesized by atom transfer radical polymerization (ATRP) of poly(ethylene glycol) methyl ether methacrylate (PEGMA), GRGDS modified poly(ethylene glycol) acrylate (GRGDS-PEG-Acryl), fluorescein o-methacrylate (FMA), and ethylene glycol dimethacrylate (EGDMA) via an "arm-first" method. Star polymers were approximately 20 nm in diameter, as measured by dynamic light scattering and atomic force microscopy. Conjugation of FMA to the stars was confirmed by fluorescence microscopy, and successful attachment of GRGDS segments to the star periphery was confirmed by (1)H NMR spectroscopy. Both fluorescent PEG star polymers with and without peripheral GRGDS peptide segments were cultured with MC3T3-E1.4 cells. These star polymers were biocompatible with ≥ 90% cell viability after 24 h of incubation. Cellular uptake of PEG star polymers in MC3T3-E1.4 cells was observed by confocal microscopy. Rapid uptake of PEG star polymers with GRGDS peptides (∼ 100% of FITC-positive cells in 15 min measured by flow cytometry) was observed, suggesting enhanced delivery potential of these functional star polymers.Keywords
This publication has 61 references indexed in Scilit:
- Nanostructured hybrid hydrogels prepared by a combination of atom transfer radical polymerization and free radical polymerizationBiomaterials, 2009
- Characterization of valvular interstitial cell function in three dimensional matrix metalloproteinase degradable PEG hydrogelsBiomaterials, 2009
- Synthesis of Maleimide-End-Functionalized Star Polymers and Multimeric Protein−Polymer ConjugatesMacromolecules, 2009
- Facile Synthesis of Multivalent Folate-Block Copolymer Conjugates via Aqueous RAFT Polymerization: Targeted Delivery of siRNA and Subsequent Gene SuppressionBiomacromolecules, 2009
- The effect of particle design on cellular internalization pathwaysProceedings of the National Academy of Sciences of the United States of America, 2008
- Peptide-Functionalized Poly(ethylene glycol) Star Polymers: DNA Delivery Vehicles with Multivalent Molecular ArchitectureBioconjugate Chemistry, 2007
- Making polymeric micro- and nanoparticles of complex shapesProceedings of the National Academy of Sciences, 2007
- Growth Factor Mediated Assembly of Cell Receptor-Responsive HydrogelsJournal of the American Chemical Society, 2007
- Core-Functionalized Star Polymers by Transition Metal-Catalyzed Living Radical Polymerization. 1. Synthesis and Characterization of Star Polymers with PMMA Arms and Amide CoresMacromolecules, 2001
- Synthesis of Star-Shaped Polystyrene by Atom Transfer Radical Polymerization Using an “Arm First” ApproachMacromolecules, 1999