Uptake of D‐serine by synaptosomal P2 fraction isolated from rat brain
- 16 August 2001
- Vol. 42 (2), 84-86
- https://doi.org/10.1002/syn.1103
Abstract
We have demonstrated that the crude synaptosome P2 fraction prepared from the rat brain is able to accumulate [3H]D-serine in a saturable, temperature-dependent and partially sodium- and potassium-dependent manner with an affinity of a hundred micromolar range. The inhibition profile of D-serine accumulation by various amino acids is different from those of uptake systems reported for glycine and other amino acids. The present data suggest that the endogenous D-serine may be taken up mainly through a carrier-mediated transport system to regulate its extracellular concentration in the mammalian brain. Synapse 42:84–86, 2001.Keywords
This publication has 14 references indexed in Scilit:
- Identification and Characterization of a Na+-independent Neutral Amino Acid Transporter That Associates with the 4F2 Heavy Chain and Exhibits Substrate Selectivity for Small Neutral d- and l-Amino AcidsJournal of Biological Chemistry, 2000
- Modulation of a Recombinant Glycine Transporter (GLYT1b) by Activation of Protein Kinase CJournal of Neurochemistry, 1995
- Glial uptake of intracerebroventricularly injected d-serine in the rat brain: an immunocytochemical studyNeuroscience Letters, 1995
- Endogenous d‐Serine in Rat Brain: N‐Methyl‐d‐Aspartate Receptor‐Related Distribution and AgingJournal of Neurochemistry, 1993
- The presence of free D‐serine in rat brainFEBS Letters, 1992
- High‐Affinity Transport of γ‐Aminobutyric Acid, Glycine, Taurine, L‐Aspartic Acid, and L‐Glutamic Acid in Synaptosomal (P2) Tissue: A Kinetic and Substrate Specificity AnalysisJournal of Neurochemistry, 1987
- Increased [3H]kainic acid binding in the prefrontal cortex in schizophreniaNeuroscience Letters, 1983
- Uptake ofl-alanine, glycine andl-serine in the pigeon central nervous systemBrain Research, 1978
- High affinity uptake systems for glycine, glutamic and aspartic acids in synaptosomes of rat central nervous tissuesBrain Research, 1972
- D-Amino Acids in AnimalsScience, 1969