The presence of free D‐serine in rat brain
- 13 January 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 296 (1), 33-36
- https://doi.org/10.1016/0014-5793(92)80397-y
Abstract
Free amino acid enantiomers in adult rat brain extracts were analysed as their N,O-pentafluoropropionyl isopropyl derivatives by gas chromatography on a capillary column of Chirasil-L-Val. A peak X, which exhibited the same retention time as the N,O-pentafluoropropionyl isopropyl derivative of authentic D-serine, was detected in the brain extracts. Electron impact and positive chemical ionization mass spectra of the peak X of the brain extracts were identical to those of authentic D-serine. The concentration of free D-serine and the ratio of D-serine/total serine in the brain were estimated to be 0.27 and 0.23, υmol/g of wet weight, respectively. These data provide the first evidence that substantial quantities of free D-serine are present in mammalian brain tissuesKeywords
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