NMR studies on the conformation of oligomannosides and their interaction with banana lectin
- 11 May 2007
- journal article
- Published by Springer Science and Business Media LLC in Glycoconjugate Journal
- Vol. 24 (8), 449-464
- https://doi.org/10.1007/s10719-007-9037-0
Abstract
The conformational and dynamic behaviour of three mannose containing oligosaccharides, a tetrasaccharide with α1→2, and α1→3, and a penta and a heptasaccharide with α1→2, α1→3, and α1→6 linkages has been evaluated by molecular mechanics and dynamics simulations and NMR spectroscopical methods. It is found that they display a fair amount of conformational freedom, with one major and one minor conformation per glycosidic linkage. The evaluation of their recognition by banana lectin has also been performed by STD NMR methods and a preliminary view of their putative interaction mode has been carried out by means of docking procedures.Keywords
This publication has 43 references indexed in Scilit:
- Unusual sugar specificity of banana lectin from Musa paradisiaca and its probable evolutionary origin. Crystallographic and modelling studiesGlycobiology, 2005
- Crystal structure of banana lectin reveals a novel second sugar binding siteGlycobiology, 2005
- Glycal Scavenging in the Synthesis of Disaccharides Using Mannosyl Iodide Donors1The Journal of Organic Chemistry, 2005
- Synthesis, Conformational Studies and Mannosidase Stability of a Mimic of 1,2‐MannobiosideEuropean Journal of Organic Chemistry, 2004
- Refinement of the Conformation of UDP−Galactose Bound to Galactosyltransferase Using the STD NMR Intensity-Restrained CORCEMA OptimizationJournal of the American Chemical Society, 2004
- Reactivity‐Based One‐Pot Synthesis of Oligomannoses: Defining Antigens Recognized by 2G12, a Broadly Neutralizing Anti‐HIV‐1 AntibodyAngewandte Chemie, 2004
- Binding of High-Mannose-Type Oligosaccharides and Synthetic Oligomannose Clusters to Human Antibody 2G12: Implications for HIV-1 Vaccine DesignCell Chemical Biology, 2004
- Lectin Structure−Activity: The Story Is Never OverJournal of Agricultural and Food Chemistry, 2002
- Semianalytical treatment of solvation for molecular mechanics and dynamicsJournal of the American Chemical Society, 1990
- A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromoleculesBiochemical and Biophysical Research Communications, 1980