Unusual sugar specificity of banana lectin from Musa paradisiaca and its probable evolutionary origin. Crystallographic and modelling studies
Open Access
- 15 June 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Glycobiology
- Vol. 15 (10), 1025-1032
- https://doi.org/10.1093/glycob/cwi087
Abstract
The crystal structure of a complex of methyl-α-D-mannoside with banana lectin from Musa paradisiaca reveals two primary binding sites in the lectin, unlike in other lectins with β-prism I fold which essentially consists of three Greek key motifs. It has been suggested that the fold evolved through successive gene duplication and fusion of an ancestral Greek key motif. In other lectins, all from dicots, the primary binding site exists on one of the three motifs in the three-fold symmetric molecule. Banana is a monocot, and the three motifs have not diverged enough to obliterate sequence similarity among them. Two Greek key motifs in it carry one primary binding site each. A common secondary binding site exists on the third Greek key. Modelling shows that both the primary sites can support 1–2, 1–3, and 1–6 linked mannosides with the second residue interacting in each case primarily with the secondary binding site. Modelling also readily leads to a bound branched mannopentose with the nonreducing ends of the two branches anchored at the two primary binding sites, providing a structural explanation for the lectin’s specificity for branched α-mannans. A comparison of the dimeric banana lectin with other β-prism I fold lectins, provides interesting insights into the variability in their quaternary structure.Keywords
This publication has 28 references indexed in Scilit:
- The Crystal Structure of the Calystegia sepium Agglutinin Reveals a Novel Quaternary Arrangement of Lectin Subunits with a β-Prism FoldPublished by Elsevier BV ,2004
- Carbohydrate binding properties of banana (Musa acuminata) lectinEuropean Journal of Biochemistry, 2001
- C-type lectin-like domainsCurrent Opinion in Structural Biology, 1999
- Helianthus tuberosus lectin reveals a widespread scaffold for mannose-binding lectinsStructure, 1999
- Crystallography & NMR System: A New Software Suite for Macromolecular Structure DeterminationActa Crystallographica Section D-Biological Crystallography, 1998
- W3-SWEET: Carbohydrate Modeling By InternetJournal of Molecular Modeling, 1998
- ALIGN: a program to superimpose protein coordinates, accounting for insertions and deletionsJournal of Applied Crystallography, 1997
- An extensively modified version of MolScript that includes greatly enhanced coloring capabilitiesJournal of Molecular Graphics and Modelling, 1997
- [25] RibbonsPublished by Elsevier BV ,1997
- The CCP4 suite: programs for protein crystallographyActa Crystallographica Section D-Biological Crystallography, 1994