A Role of Fluoride on Free Radical Generation and Oxidative Stress in BV-2 Microglia Cells
Open Access
- 13 August 2012
- journal article
- research article
- Published by Hindawi Limited in Mediators of Inflammation
- Vol. 2012, 1-8
- https://doi.org/10.1155/2012/102954
Abstract
The generation of ROS and lipid peroxidation has been considered to play an important role in the pathogenesis of chronic fluoride toxicity. In the present study, we observed that fluoride activated BV-2 microglia cell line by observing OX-42 expression in immunocytochemistry. Intracellular superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), reactive oxygen species (ROS), superoxide anions (), nitric oxide synthase (NOS), nitrotyrosine (NT) and nitric oxide (NO), NOS in cell medium were determined for oxidative stress assessment. Our study found that NaF of concentration from 5 to 20 mg/L can stimuli BV-2 cells to change into activated microglia displaying upregulated OX-42 expression. SOD activities significantly decreased in fluoride-treated BV-2 cells as compared with control, and MDA concentrations and contents of ROS andincreased in NaF-treated cells. Activities of NOS in cells and medium significantly increased with fluoride concentrations in a dose-dependent manner. NT concentrations also increased significantly in 10 and 50 mg/L NaF-treated cells compared with the control cells. Our present study demonstrated that toxic effects of fluoride on the central nervous system possibly partly ascribed to activiting of microglia, which enhanced oxidative stress induced by ROS and reactive nitrogen species.
Keywords
Funding Information
- Ministry of Education, Science and Technology Development Center of China (20112104110021)
This publication has 48 references indexed in Scilit:
- Proliferative Neural Stem Cells Have High Endogenous ROS Levels that Regulate Self-Renewal and Neurogenesis in a PI3K/Akt-Dependant MannerCell Stem Cell, 2011
- Revisiting oxidative damage in ALS: microglia, Nox, and mutant SOD1JCI Insight, 2008
- Reactive oxygen species and p47phox activation are essential for the Mycobacterium tuberculosis-induced pro-inflammatory response in murine microgliaJournal of Neuroinflammation, 2007
- Arsenic and Fluoride Exposure in Drinking Water: Children’s IQ and Growth in Shanyin County, Shanxi Province, ChinaEnvironmental Health Perspectives, 2007
- Oxidative stress induced by fluoride in adult mice and their suckling pupsExperimental and Toxicologic Pathology, 2007
- Activation of NADPH oxidase and extracellular superoxide production in seizure‐induced hippocampal damageJournal of Neurochemistry, 2004
- Cytotoxicity of microgliaGlia, 1993
- Red blood cell glucose metabolism in human chronic fluoride toxicityBulletin of Environmental Contamination and Toxicology, 1991
- Production of superoxide anions by a CNS macrophage, the microgliaFEBS Letters, 1987
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976