Cancer chemoprevention by hydroxytyrosol isolated from virgin olive oil through G1 cell cycle arrest and apoptosis
- 1 August 2002
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in European Journal of Cancer Prevention
- Vol. 11 (4), 351-358
- https://doi.org/10.1097/00008469-200208000-00006
Abstract
Recent epidemiological evidence and animal studies suggest a relationship between the intake of olive oil and a reduced risk of several malignancies. The present study assesses the effect of hydroxytyrosol, a major antioxidant compound of virgin olive oil, on proliferation, apoptosis and cell cycle of tumour cells. Hydroxytyrosol inhibited proliferation of both human promyelocytic leukaemia cells HL60 and colon adenocarcinoma cells HT29 and HT29 clone 19A. The con-centrations of hydroxytyrosol which inhibited 50% of cell proliferation were ∼50 and ∼750 μmol/l for HL60 and both HT29 and HT29 clone 19A cells, respectively. At concentrations ranging from 50 to 100 μmol/l, hydroxytyrosol induced an appreciable apoptosis in HL60 cells after 24 h of incubation as evidenced by flow cytometry, fluorescence microscopy and internucleosomal DNA fragmentation. Interestingly, no effect on apoptosis was observed after similar treatment of freshly isolated human lymphocytes and polymorphonuclear cells. The DNA cell cycle analysis, quantified by flow cytometry, showed that the treatment of HL60 cells with hydroxytyrosol 50–100 μmol/l arrested the cells in the G0/G1 phase with a concomitant decrease in the cell percentage in the S and G2/M phases. These results support the hypothesis that hydroxytyrosol may exert a protective activity against cancer by arresting the cell cycle and inducing apoptosis in tumour cells, and suggest that hydroxytyrosol, an important component of virgin olive oil, may be responsible for its anticancer activity.Keywords
This publication has 35 references indexed in Scilit:
- Hydroxytyrosol, a Natural Molecule Occurring in Olive Oil, Induces Cytochrome c-Dependent ApoptosisBiochemical and Biophysical Research Communications, 2000
- Effect of olive oil on early and late events of colon carcinogenesis in rats: modulation of arachidonic acid metabolism and local prostaglandin E2 synthesisGut, 2000
- Inhibition of peroxynitrite dependent DNA base modification and tyrosine nitration by the extra virgin olive oil-derived antioxidant hydroxytyrosolFree Radical Biology & Medicine, 1999
- Olive oil, other seasoning fats, and the risk of colorectal carcinomaCancer, 1998
- Antioxidant activity of tocopherols and phenolic compounds of virgin olive oilJournal of Oil & Fat Industries, 1996
- Glucose promotes survival of rat pancreatic beta cells by activating synthesis of proteins which suppress a constitutive apoptotic program.JCI Insight, 1996
- Cyclins and cancer II: Cyclin D and CDK inhibitors come of ageCell, 1994
- Protection of low-density lipoprotein from oxidation by 3,4-dihydroxyphenylethanolThe Lancet, 1994
- A new approach for measurement of cytotoxicity using colorimetric assayJournal of Immunological Methods, 1993
- Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometryBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1992