Expression of a Calcium-Mobilizing Parathyroid Hormone-Like Peptide in Lactating Mammary Tissue
- 14 October 1988
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 242 (4876), 278-280
- https://doi.org/10.1126/science.3175653
Abstract
A survey of rat tissues by RNA analysis, aimed at uncovering the physiological function of the parathyroid hormone-like peptide (PTH-LP) associated with hypercalcemia of malignancy, revealed the presence of a 1.5-kilobase messenger RNA encoding this peptide in lactating mammary glands. PTH-LP messenger RNA is expressed in mammary tissue only during lactation; it appears and disappears rapidly (2 to 4 hours) as a function of the sucking stimulus. The identity of this messenger RNA was confirmed by cloning the rat PTH-LP complementary DNA, which predicts a peptide with strong similarity to the human homolog. Moreover, extracts from lactating mammary tissue stimulated parathyroid hormone-dependent adenylate cyclase. These findings suggest that PTH-LP plays a physiological role in lactation, possibly as a hormone for the mobilization or transfer (or both) of calcium to the milk.Keywords
This publication has 21 references indexed in Scilit:
- Parathyroid Hormone-Related Protein of Malignancy: Active Synthetic FragmentsScience, 1987
- Similarity of Synthetic Peptide from Human Tumor to Parathyroid Hormone in Vivo and in VitroScience, 1987
- Parathyroid hormonelike protein from human renal carcinoma cells. Structural and functional homology with parathyroid hormone.JCI Insight, 1987
- A Parathyroid Hormone-Related Protein Implicated in Malignant Hypercalcemia: Cloning and ExpressionScience, 1987
- N-Terminal amino acid sequence of two novel tumor-derived adenylate cyclase-stimulating proteins: Identification of parathyroid hormone-like and parathyroid hormone-unlike domainsBiochemical and Biophysical Research Communications, 1987
- A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradationCell, 1986
- Factors associated with humoral hypercalcemia of malignancy stimulate adenylate cyclase in osteoblastic cells.JCI Insight, 1983
- Role of prolactin in vitamin D metabolism and calcium absorption during lactation in the ratJournal of Endocrinology, 1982
- Stimulation of Intestinal Calcium Transport and Bone Calcium Mobilization by Prolactin in Vitamin D-Deficient RatsScience, 1981
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979