New Aldehyde Tag Sequences Identified by Screening Formylglycine Generating Enzymes in Vitro and in Vivo
Open Access
- 23 August 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 130 (37), 12240-12241
- https://doi.org/10.1021/ja804530w
Abstract
Formylglycine generating enzyme (FGE) performs a critical posttranslational modification of type I sulfatases, converting cysteine within the motif CxPxR to the aldehyde-bearing residue formylglycine (FGly). This concise motif can be installed within heterologous proteins as a genetically encoded “aldehyde tag” for site-specific labeling with aminooxy- or hydrazide-functionalized probes. In this report, we screened FGEs from M. tuberculosis and S. coelicolor against synthetic peptide libraries and identified new substrate sequences that diverge from the canonical motif. We found that E. coli’s FGE-like activity is similarly promiscuous, enabling the use of novel aldehyde tag sequences for in vivo modification of recombinant proteins.Keywords
This publication has 10 references indexed in Scilit:
- Function and Structure of a Prokaryotic Formylglycine-generating EnzymePublished by Elsevier BV ,2008
- Introducing genetically encoded aldehydes into proteinsNature Chemical Biology, 2007
- Anaerobic Sulfatase-Maturating Enzymes: Radical SAM Enzymes Able To Catalyze in Vitro Sulfatase Post-translational ModificationJournal of the American Chemical Society, 2007
- A New Type of Bacterial Sulfatase Reveals a Novel Maturation Pathway in ProkaryotesJournal of Biological Chemistry, 2006
- A general binding mechanism for all human sulfatases by the formylglycine-generating enzymeProceedings of the National Academy of Sciences of the United States of America, 2005
- Multiple Sulfatase Deficiency Is Caused by Mutations in the Gene Encoding the Human Cα-Formylglycine Generating EnzymeCell, 2003
- The Iron Sulfur Protein AtsB Is Required for Posttranslational Formation of Formylglycine in the Klebsiella SulfataseJournal of Biological Chemistry, 1999
- Sequence determinants directing conversion of cysteine to formylglycine in eukaryotic sulfatasesThe EMBO Journal, 1999
- Residues Critical for Formylglycine Formation and/or Catalytic Activity of Arylsulfatase ABiochemistry, 1998
- A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiencyCell, 1995