Introducing genetically encoded aldehydes into proteins
Top Cited Papers
- 22 April 2007
- journal article
- Published by Springer Science and Business Media LLC in Nature Chemical Biology
- Vol. 3 (6), 321-322
- https://doi.org/10.1038/nchembio878
Abstract
Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications.Keywords
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