Molecular Cloning of Human Alpha and Beta Interferon Genes from Namalwa Cells
- 1 January 1982
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Interferon Research
- Vol. 2 (4), 575-585
- https://doi.org/10.1089/jir.1982.2.575
Abstract
A cDNA library constructed from poly(A)+RNA of Sendai virus-induced human lymphoblastoid (Namalwa) cells was screened with a synthetic oligonucleotide specific for interferon genes. Recombinant plasmids containing sequences derived from alpha and beta interferon (IFN) mRNAs were obtained. The clones were characterized by RNA transfer hybridization, translation of hybrid-isolated RNA, and DNA sequencing. One alpha interferon (IFN-α) clone obtained is a variant differing from previously described clones in the location of its polyadenylation site.Keywords
This publication has 41 references indexed in Scilit:
- Molecular analysis of the human interferon-α gene familyGene, 1981
- Production of high levels of human leukocyte interferon from a continuous human myeloblast cell cultureAntimicrobial Agents and Chemotherapy, 1981
- The structure of eight distinct cloned human leukocyte interferon cDNAsNature, 1981
- Human leukocyte interferon produced by E. coli is biologically activeNature, 1980
- The structure of one of the eight or more distinct chromosomal genes for human interferon-αNature, 1980
- At Least Three Human Type α Interferons: Structure of α2Science, 1980
- Synthesis in E. coli of a polypeptide with human leukocyte interferon activityNature, 1980
- Characteristics of Human Lymphoblastoid (Namalva) InterferonJournal of General Virology, 1978
- Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.Proceedings of the National Academy of Sciences of the United States of America, 1977
- Two antigenically distinct species of human interferon.Proceedings of the National Academy of Sciences of the United States of America, 1975