NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons
- 4 July 2013
- journal article
- Published by Wiley in FEBS Letters
- Vol. 587 (16), 2643-2648
- https://doi.org/10.1016/j.febslet.2013.06.049
Abstract
Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activationKeywords
Funding Information
- Scientific Research (A) (24240045)
- Strategic Research Foundation Grant-aided Project for Private Universities from Ministry of Education (S0801008)
- Culture, Sport, Science, and Technology, Japan (MEXT)
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