A nanobody targeting the F-actin capping protein CapG restrains breast cancer metastasis
Open Access
- 13 December 2013
- journal article
- Published by Springer Science and Business Media LLC in Breast Cancer Research
- Vol. 15 (6), R116
- https://doi.org/10.1186/bcr3585
Abstract
Aberrant turnover of the actin cytoskeleton is intimately associated with cancer cell migration and invasion. Frequently however, evidence is circumstantial, and a reliable assessment of the therapeutic significance of a gene product is offset by lack of inhibitors that target biologic properties of a protein, as most conventional drugs do, instead of the corresponding gene. Proteomic studies have demonstrated overexpression of CapG, a constituent of the actin cytoskeleton, in breast cancer. Indirect evidence suggests that CapG is involved in tumor cell dissemination and metastasis. In this study, we used llama-derived CapG single-domain antibodies or nanobodies in a breast cancer metastasis model to address whether inhibition of CapG activity holds therapeutic merit.Keywords
This publication has 48 references indexed in Scilit:
- From filopodia to synapses: the role of actin-capping and anti-capping proteinsEuropean Journal of Neuroscience, 2011
- Crystal structure of the β2 adrenergic receptor–Gs protein complexNature, 2011
- The 'ins' and 'outs' of podosomes and invadopodia: characteristics, formation and functionNature Reviews Molecular Cell Biology, 2011
- Migrastatin analogues target fascin to block tumour metastasisNature, 2010
- Actin, a Central Player in Cell Shape and MovementScience, 2009
- Characterization of two classes of small molecule inhibitors of Arp2/3 complexNature, 2009
- Complete Genome Sequence and Comparative Genome Analysis of Enteropathogenic Escherichia coli O127:H6 Strain E2348/69Journal of Bacteriology, 2009
- Pancreatic cancer cells overexpress gelsolin family-capping proteins, which contribute to their cell motilityGut, 2007
- Protein delivery into eukaryotic cells by type III secretion machinesNature, 2006
- A CapG gain-of-function mutant reveals critical structural and functional determinants for actin filament severingThe EMBO Journal, 2006