T‐Cell Phenotypes Predictive of Frailty and Mortality in Elderly Nursing Home Residents
- 24 October 2016
- journal article
- research article
- Published by Wiley in Journal of the American Geriatrics Society
- Vol. 65 (1), 153-159
- https://doi.org/10.1111/jgs.14507
Abstract
Objectives To determine whether immune phenotypes associated with immunosenescence are predictive of frailty and mortality within 1‐year in elderly nursing home residents. Design Cross sectional study of frailty; prospective cohort study of mortality. Setting Thirty‐two nursing homes in four Canadian cities between September 2009 and October 2011. Participants Nursing home residents aged 65 and older (N = 1,072, median age 86, 72% female). Measurements After enrollment, peripheral blood mononuclear cells were obtained and analyzed using flow cytometry for CD4+ and CD8+ T‐cell subsets (naïve, memory (central, effector, terminally differentiated, senescent), and regulatory T‐cells) and cytomegalovirus (CMV)‐reactive CD4+ and CD8+ T‐cells. Multilevel linear regression analysis was performed to determine the relationship between immune phenotypes and frailty; frailty was measured at the time of enrollment using the Frailty Index. A Cox proportional hazards model was used to determine the relationship between immune phenotypes and time to death (within 1 year). Results Mean Frailty Index was 0.44 ± 0.13. Multilevel regression analysis showed that higher percentages of naïve CD4+ T‐cells (P = .001) and effector memory CD8+ T‐cells (P = .02) were associated with a lower mean Frailty Index, whereas a higher percentage of CD8+ central memory T‐cells was associated with a higher mean Frailty Index score (P = .02). One hundred fifty one (14%) members of the cohort died within 1 year. Multivariable analysis showed a significant negative multiplicative interaction between age and percentage of CMV‐reactive CD4+ T‐cells (hazard ratio = 0.87, 95% confidence interval = 0.79–0.96). No other significant factors were identified. Conclusion Immune phenotypes found to be predictive of frailty and mortality in this study can help further understanding of immunosenescence and may provide a rationale for future intervention studies designed to modulate immunity.Keywords
Funding Information
- Canadian Institutes of Health Research
- Public Health Agency
- National Institutes of Health (R01 EB008400/EB/NIBIB)
- Natural Sciences and Engineering Research Council of Canada
This publication has 40 references indexed in Scilit:
- Thymic function failure and C-reactive protein levels are independent predictors of all-cause mortality in healthy elderly humansAGE, 2011
- Senescence of the Human Immune SystemJournal of Comparative Pathology, 2009
- Accumulation of memory T cells from childhood to old age: Central and effector memory cells in CD4+ versus effector memory and terminally differentiated memory cells in CD8+ compartmentMechanisms of Ageing and Development, 2006
- Dysregulation of T-Cell Function in the ElderlyDrugs & Aging, 2005
- Expansions of peripheral blood CD8 T-lymphocyte subpopulations and an association with cytomegalovirus seropositivity in the elderly: the Swedish NONA immune studyExperimental Gerontology, 2002
- Age-related change in peripheral blood T-lymphocyte subpopulations and cytomegalovirus infection in the very old: the Swedish longitudinal OCTO immune studyMechanisms of Ageing and Development, 2001
- Distinct categories of immunologic changes in frail elderlyMechanisms of Ageing and Development, 2000
- CD28 Expression in T Cell Aging and Human LongevityExperimental Gerontology, 1998
- Changes in CD8 and CD4 lymphocyte subsets, T cell proliferation responses and non-survival in the very old: the Swedish longitudinal OCTO-immune studyMechanisms of Ageing and Development, 1998
- Decline in CD28+ T cells in centenarians and in long-term T cell cultures: A possible cause for both in vivo and in vitro immunosenescenceExperimental Gerontology, 1994