Degradation of phenanthrene and naphthalene by aBurkholderiaspecies strain
- 1 February 2003
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 49 (2), 139-144
- https://doi.org/10.1139/w03-009
Abstract
Burkholderia sp. TNFYE-5 was isolated from soil for the ability to grow on phenanthrene as sole carbon and energy source. Unlike most other phenanthrene-degrading bacteria, TNFYE-5 was unable to grow on naphthalene. Growth substrate range experiments coupled with the ring-cleavage enzyme assay data suggest that TNFYE-5 initially metabolizes phenanthrene to 1-hydroxy-2-naphthoate with subsequent degradation through the phthalate and protocatechuate and β-ketoadipate pathway. A metabolite in the degradation of naphthalene by TNFYE-5 was isolated by high-pressure liquid chromatography (HPLC) and was identified as salicylate by UV-visible spectral and gas chromatography – mass spectrometry analyses. Thus, the inability to degrade salicylate is apparently one major reason for the incapability of TNFYE-5 to grow on naphthalene.Key words: Burkholderia, phenanthrene, naphthalene, phthalate, protocatechuate.Keywords
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