Novel immunogenic antigens increase classification accuracy in meningioma to 93.84%
Open Access
- 28 January 2011
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 128 (6), 1493-1501
- https://doi.org/10.1002/ijc.25467
Abstract
There is growing evidence that simultaneous analysis of multiple autoantibody reactions can be utilized for diagnosis of neoplasms. Using a set of 57 meningioma‐associated antigens, we recently separated meningioma patients from individuals without known disease with an accuracy of 90.3%. Here, we ask whether a largely increased set of immunogenic antigens can further improve this discrimination. We used an array with 1,827 human recombinant clones and measured reactivity of serum autoantibodies against the clones by a novel automated image analysis procedure. We were able to separate meningioma sera from sera of healthy controls with a specificity of 95.62%, a sensitivity of 91.83% and an accuracy of 93.84%. Of the analyzed clones, 23 in‐frame clones were highly informative for the classification of meningioma vs. normal sera as shown by their AUC values. These results demonstrate that the accuracy of a serum‐based diagnostic can be readily and considerably improved by screening extended sets of proteins.Keywords
This publication has 35 references indexed in Scilit:
- Meningiomas in 2009American Journal of Clinical Oncology, 2009
- Molecular pathogenesis of meningiomasJournal of Neuro-Oncology, 2004
- Immunohistochemical analysis of p16INK4a, p14ARF, p18INK4c, p21CIP1, p27KIP1 and p73 expression in 271 meningiomas correlation with tumor grade and clinical outcomeInternational Journal of Cancer, 2003
- A Role for Chromosome 9p21 Deletions in the Malignant Progression of Meningiomas and the Prognosis of Anaplastic MeningiomasBrain Pathology, 2002
- Alterations of the Tumor Suppressor Genes CDKN2A (p16INK4a), p14ARF, CDKN2B (p15INK4b), and CDKN2C (p18INK4c) in Atypical and Anaplastic MeningiomasThe American Journal of Pathology, 2001
- INI1 mutations in meningiomas at a potential hotspot in exon 9British Journal of Cancer, 2001
- The human LARGE gene from 22q12.3-q13.1 is a new, distinct member of the glycosyltransferase gene familyProceedings of the National Academy of Sciences of the United States of America, 1999
- Molecular genetic investigation of the neurofibromatosis type 2 tumor suppressor gene in sporadic meningiomaJournal of Neurosurgery, 1996
- Characterization of a new member of the human /-adaptin gene family from chromosome 22q12, a candidate meningioma geneHuman Molecular Genetics, 1994
- Evidence for the complete inactivation of the NF2 gene in the majority of sporadic meningiomasNature Genetics, 1994