Characterization of a new member of the human /-adaptin gene family from chromosome 22q12, a candidate meningioma gene

Abstract

A 140 kb homozygous deletion from 22q12 In one menlngioma directed us towards the cloning and characterization of a new member of the human β-adaptln gene family (named BAM22). Adaptins are essential for the formation of clathrin coated vesicles in the course of intracellular transport of receptor-ligand complexes. The BAM22 gene is totally inactivated In the tumor with homozygous deletion. Northern blot analysis of 70 sporadic meningiomas showed specific loss of expression in 8 tumors, suggesting inactivation of BAM22. Based on this, we propose BAM22 as a second chromosome 22 locus Important in meningioma development, after the neuroflbromatosis type 2 gene.