Isolation of Viral IgY Antibodies from Yolks of Immunized Hens
- 1 January 1980
- journal article
- research article
- Published by Taylor & Francis Ltd in Immunological Communications
- Vol. 9 (5), 475-493
- https://doi.org/10.3109/08820138009066010
Abstract
Antibodies were isolated from the yolks of hens that were immunized with a variety of plant viruses by the use of polyethylene glycol (PEG). A concentration of 3.5% of the polymer caused the lipids and vitellin to separate, and the IgY was then precipitated with 12% PEG. The titre of the isolated antibody appears to remain at a high level after cessation of the course of immunization. Antibodies derived from the yolks of hens appear to have titres similar to those found in serum of rabbits immunized simultaneously. The observation made by several authors that a high salt concentration enhances fowl serum antibody precipitin titres could not be corroborated with ‘yolk’ antibodies directed against several plant viruses.Keywords
This publication has 7 references indexed in Scilit:
- Isolation of specific antibody under conditions of low ionic strengthJournal of Immunological Methods, 1977
- PHYLOGENY OF IMMUNOGLOBULIN STRUCTURE AND FUNCTIONThe Journal of Experimental Medicine, 1969
- Molecular weights of chicken, pheasant, and quail IgG immunoglobulinsImmunochemistry, 1969
- Serum proteins and the livetins of hen's-egg yolkBiochemical Journal, 1962
- Serological studies of the blood of the developing chick embryoJournal of Experimental Zoology, 1953
- STUDIES OF THE COMPOSITION OF THE LIVETIN FRACTION OF THE YOLK OF HEN'S EGGS WITH THE USE OF ELECTROPHORETIC ANALYSISPublished by Elsevier BV ,1949
- A Demonstration of Some New Methods of Determining Molecular Weights from the Data of the Ultracentrifuge.The Journal of Physical Chemistry, 1947