Viral and cellular requirements for the budding of Feline Endogenous Retrovirus RD-114
Open Access
- 14 December 2011
- journal article
- Published by Springer Science and Business Media LLC in Virology Journal
- Vol. 8 (1), 540
- https://doi.org/10.1186/1743-422x-8-540
Abstract
Background RD-114 virus is a feline endogenous retrovirus and produced as infectious viruses in some feline cell lines. Recently, we reported the contamination of an infectious RD-114 virus in a proportion of live attenuated vaccines for dogs and cats. It is very difficult to completely knock out the RD-114 proviruses from cells, as endogenous retroviruses are usually integrated multiply into the host genome. However, it may be possible to reduce the risk of contamination of RD-114 virus by regulating the viral release from cells. Results In this study, to understand the molecular mechanism of RD-114 virus budding, we attempted to identify the viral and cellular requirements for RD-114 virus budding. Analyses of RD-114 L-domain mutants showed that the PPPY sequence in the pp15 region of Gag plays a critical role in RD-114 virus release as viral L-domain. Furthermore, we investigated the cellular factors required for RD-114 virus budding. We demonstrated that RD-114 virus release was inhibited by overexpression of dominant negative mutants of Vps4A, Vps4B, and WWP2. Conclusions These results strongly suggest that RD-114 budding utilizes the cellular multivesicular body sorting pathway similar to many other retroviruses.Keywords
This publication has 30 references indexed in Scilit:
- Cloning and Characterization of the Antiviral Activity of Feline Tetherin/BST-2PLOS ONE, 2011
- Susceptibility and production of a feline endogenous retrovirus (RD-114 virus) in various feline cell linesVirus Research, 2011
- Characterization of RD-114 Virus Isolated from a Commercial Canine Vaccine Manufactured Using CRFK CellsJournal of Clinical Microbiology, 2010
- Isolation of an Infectious Endogenous Retrovirus in a Proportion of Live Attenuated Vaccines for PetsJournal of Virology, 2010
- The HECT-Type E3 Ubiquitin Ligase AIP2 Inhibits Activation-Induced T-Cell Death by Catalyzing EGR2 UbiquitinationMolecular and Cellular Biology, 2009
- Vacuolar Protein Sorting Pathway Contributes to the Release of Marburg VirusJournal of Virology, 2009
- Inhibition of Lassa and Marburg Virus Production by TetherinJournal of Virology, 2009
- Interaction of Tsg101 with Marburg Virus VP40 Depends on the PPPY Motif, but Not the PT/SAP Motif as in the Case of Ebola Virus, and Tsg101 Plays a Critical Role in the Budding of Marburg Virus-Like Particles Induced by VP40, NP, and GPJournal of Virology, 2007
- THE ESCRT COMPLEXES: Structure and Mechanism of a Membrane-Trafficking NetworkAnnual Review of Biophysics and Biophysical Chemistry, 2006
- Cellular Factors Required for Lassa Virus BuddingJournal of Virology, 2006