Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression
Open Access
- 15 July 2015
- journal article
- for the-record
- Published by Wiley in Protein Science
- Vol. 24 (10), 1695-1701
- https://doi.org/10.1002/pro.2748
Abstract
We recently reported the development of a computational method for the design of coassembling multicomponent protein nanomaterials. While four such materials were validated at high-resolution by X-ray crystallography, low yield of soluble protein prevented X-ray structure determination of a fifth designed material, T33-09. Here we report the design and crystal structure of T33-31, a variant of T33-09 with improved soluble yield resulting from redesign efforts focused on mutating solvent-exposed side chains to charged amino acids. The structure is found to match the computational design model with atomic-level accuracy, providing further validation of the design approach and demonstrating a simple and potentially general means of improving the yield of designed protein nanomaterials.Keywords
Funding Information
- National Science Foundation (CHE-1332907, DGE-0718124)
- Defense Advanced Research Projects Agency (W911NF-14-1-0162)
- Howard Hughes Medical Institute, JFRC visitor program
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