Amorphous nanosilica induce endocytosis-dependent ROS generation and DNA damage in human keratinocytes
Open Access
- 15 January 2011
- journal article
- Published by Springer Science and Business Media LLC in Particle and Fibre Toxicology
- Vol. 8 (1), 1
- https://doi.org/10.1186/1743-8977-8-1
Abstract
Clarifying the physicochemical properties of nanomaterials is crucial for hazard assessment and the safe application of these substances. With this in mind, we analyzed the relationship between particle size and the in vitro effect of amorphous nanosilica (nSP). Specifically, we evaluated the relationship between particle size of nSP and the in vitro biological effects using human keratinocyte cells (HaCaT). Our results indicate that exposure to nSP of 70 nm diameter (nSP70) induced an elevated level of reactive oxygen species (ROS), leading to DNA damage. A markedly reduced response was observed using submicron-sized silica particles of 300 and 1000 nm diameter. In addition, cytochalasin D-treatment reduced nSP70-mediated ROS generation and DNA damage, suggesting that endocytosis is involved in nSP70-mediated cellular effects. Thus, particle size affects amorphous silica-induced ROS generation and DNA damage of HaCaT cells. We believe clarification of the endocytosis pathway of nSP will provide useful information for hazard assessment as well as the design of safer forms of nSPs.Keywords
This publication has 64 references indexed in Scilit:
- Quantitative Nanostructure−Activity Relationship ModelingACS Nano, 2010
- Nanomaterial cytotoxicity is composition, size, and cell type dependentParticle and Fibre Toxicology, 2010
- Particulate Matter Disrupts Human Lung Endothelial Barrier Integrity via ROS- and p38 MAPK–Dependent PathwaysAmerican Journal of Respiratory Cell and Molecular Biology, 2010
- Effects of nanomaterial physicochemical properties on in vivo toxicityAdvanced Drug Delivery Reviews, 2009
- Macrophage Responses to Silica Nanoparticles are Highly Conserved Across Particle SizesToxicological Sciences, 2008
- Acute Toxicity and Prothrombotic Effects of Quantum Dots: Impact of Surface ChargeEnvironmental Health Perspectives, 2008
- Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilizationNature Immunology, 2008
- Perturbational profiling of nanomaterial biologic activityProceedings of the National Academy of Sciences of the United States of America, 2008
- Innate Immune Activation Through Nalp3 Inflammasome Sensing of Asbestos and SilicaScience, 2008
- Endosomal NADPH oxidase regulates c-Src activation following hypoxia/reoxygenation injuryBiochemical Journal, 2008