Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif
Open Access
- 26 October 1999
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 96 (22), 12424-12429
- https://doi.org/10.1073/pnas.96.22.12424
Abstract
Surface proteins of Staphylococcus aureus are linked to the bacterial cell wall by sortase, an enzyme that cleaves polypeptides at the threonine of the LPXTG motif. Surface proteins can be released from staphylococci by treatment with hydroxylamine, resulting in the formation of threonine hydroxamate. Staphylococcal extracts, as well as purified sortase, catalyze the hydroxylaminolysis of peptides bearing an LPXTG motif, a reaction that can be inhibited with sulfhydryl-modifying reagents. Replacement of the single conserved cysteine at position 184 of sortase with alanine abolishes enzyme activity. Thus, sortase appears to catalyze surface-protein anchoring by means of a transpeptidation reaction that captures cleaved polypeptides as thioester enzyme intermediates.Keywords
This publication has 28 references indexed in Scilit:
- Staphylococcus aureus Sortase, an Enzyme that Anchors Surface Proteins to the Cell WallScience, 1999
- Multiple Enzymatic Activities of the Murein Hydrolase from Staphylococcal Phage φ11Published by Elsevier BV ,1999
- Anchor Structure of Staphylococcal Surface ProteinsPublished by Elsevier BV ,1998
- Protein Splicing: Evidence for an N−O Acyl Rearrangement as the Initial Step in the Splicing ProcessBiochemistry, 1996
- Structure of the Cell Wall Anchor of Surface Proteins in Staphylococcus aureusScience, 1995
- Sulfhydryl Modification of the Yeast Wbp1p Inhibits Oligosaccharyl Transferase ActivityBiochemistry, 1995
- Sorting of protein a to the staphylococcal cell wallCell, 1992
- SERINE β-LACTAMASES AND PENICILLIN-BINDING PROTEINSAnnual Review of Microbiology, 1991
- Novel Fluorogenic Substrates for Assaying Retroviral Proteases by Resonance Energy TransferScience, 1990
- PENICILLIN-BINDING PROTEINS AND THE MECHANISM OF ACTION OF BETA-LACTAM ANTIBIOTICSAnnual Review of Biochemistry, 1983