Staphylococcus aureus Sortase, an Enzyme that Anchors Surface Proteins to the Cell Wall
- 30 July 1999
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 285 (5428), 760-763
- https://doi.org/10.1126/science.285.5428.760
Abstract
Surface proteins of Gram-positive bacteria are linked to the bacterial cell wall by a mechanism that involves cleavage of a conserved Leu-Pro-X-Thr-Gly (LPXTG) motif and that occurs during assembly of the peptidoglycan cell wall. A Staphylococcus aureus mutant defective in the anchoring of surface proteins was isolated and shown to carry a mutation in the srtA gene. Overexpression of srtA increased the rate of surface protein anchoring, and homologs of srtA were found in other pathogenic Gram-positive bacteria. The protein specified by srtA , sortase, may be a useful target for the development of new antimicrobial drugs.Keywords
This publication has 15 references indexed in Scilit:
- Multiple Enzymatic Activities of the Murein Hydrolase from Staphylococcal Phage φ11Journal of Biological Chemistry, 1999
- The Development of Vancomycin Resistance in a Patient with Methicillin-ResistantStaphylococcus aureusInfectionNew England Journal of Medicine, 1999
- Anchor Structure of Staphylococcal Surface ProteinsJournal of Biological Chemistry, 1998
- Antimicrobial-Drug ResistanceNew England Journal of Medicine, 1996
- Staphylococcal Peptidoglycan Interpeptide Bridge Biosynthesis: A Novel Antistaphylococcal Target?Microbial Drug Resistance, 1996
- Structure of the Cell Wall Anchor of Surface Proteins in Staphylococcus aureusScience, 1995
- Expression of resistance to methicillinTrends in Microbiology, 1994
- Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in Gram‐positive bacteriaMolecular Microbiology, 1994
- Vancomycin Resistance: Decoding the Molecular LogicScience, 1993
- Sorting of protein a to the staphylococcal cell wallCell, 1992