Applications of Digital PCR for Clinical Microbiology
- 1 June 2017
- journal article
- review article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 55 (6), 1621-1628
- https://doi.org/10.1128/jcm.00211-17
Abstract
Digital PCR (dPCR) is an important new tool for use in the clinical microbiology laboratory. Its advantages over quantitative PCR (qPCR), including absolute quantification without a standard curve, improved precision, improved accuracy in the presence of inhibitors, and more accurate quantitation when amplification efficiency is low, make dPCR the assay of choice for several specimen testing applications. This minireview will discuss the advantages and disadvantages of dPCR compared to qPCR, its applications in clinical microbiology, and considerations for implementation of the method in a clinical laboratory.Keywords
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