The potential advantages of digital PCR for clinical virology diagnostics
- 11 April 2014
- journal article
- review article
- Published by Taylor & Francis Ltd in Expert Review of Molecular Diagnostics
- Vol. 14 (4), 501-507
- https://doi.org/10.1586/14737159.2014.910456
Abstract
Digital PCR (dPCR), a new nucleic acid amplification technology, offers several potential advantages over real-time or quantitative PCR (qPCR), the current workhorse of clinical molecular virology diagnostics. Several studies have demonstrated dPCR assays for human cytomegalovirus or HIV, which give more precise and reproducible results than qPCR assays without sacrificing sensitivity. Here we review the literature comparing dPCR and qPCR performance in viral molecular diagnostic assays and offer perspective on the future of dPCR in clinical virology diagnostics.Keywords
This publication has 30 references indexed in Scilit:
- Viral diagnostics in the era of digital polymerase chain reactionDiagnostic Microbiology and Infectious Disease, 2012
- Digital PCR hits its strideNature Methods, 2012
- Factors Contributing to Variability of Quantitative Viral PCR Results in Proficiency Testing Samples: a Multivariate AnalysisJournal of Clinical Microbiology, 2012
- Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number QuantificationAnalytical Chemistry, 2011
- High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy NumberAnalytical Chemistry, 2011
- Comparison of methods for extraction of viral DNA from cellular specimensDiagnostic Microbiology and Infectious Disease, 2009
- Multicenter Comparison of Different Real-Time PCR Assays for Quantitative Detection of Epstein-Barr VirusJournal of Clinical Microbiology, 2008
- Optimization of Quantitative Detection of Cytomegalovirus DNA in Plasma by Real-Time PCRJournal of Clinical Microbiology, 2004
- Evaluation of Real-Time PCR versus PCR with Liquid-Phase Hybridization for Detection of Enterovirus RNA in Cerebrospinal FluidJournal of Clinical Microbiology, 2003
- Digital PCRProceedings of the National Academy of Sciences of the United States of America, 1999