Substantial Alterations of the Cutaneous Bacterial Biota in Psoriatic Lesions
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Open Access
- 23 July 2008
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 3 (7), e2719
- https://doi.org/10.1371/journal.pone.0002719
Abstract
For psoriasis, an idiopathic inflammatory disorder of the skin, the microbial biota has not been defined using cultivation-independent methods. We used broad-range 16S rDNA PCR for archaea and bacteria to examine the microbiota of normal and psoriatic skin. From 6 patients, 19 cutaneous samples (13 from diseased skin and 6 from normal skin) were obtained. Extracted DNA was subjected to the broad range PCR, and 1,925 cloned products were compared with 2,038 products previously reported from healthy persons. Using 98% sequence identity as a species boundary, 1,841 (95.6%) clones were similar to known bacterial 16S rDNA, representing 6 phyla, 86 genera, or 189 species-level operational taxonomic unit (SLOTU); 84 (4.4%) clones with Firmicutes (46.2%), significantly (PActinobacteria, the most prevalent and diverse phylum in normal skin samples from both healthy persons (47.6%) and the patients (47.8%), was significantly (PPropionibacterium species were lower in the psoriatic lesions (2.9±5.5%) than from normal persons (21.1±18.2%; P<0.001), whereas normal skin from the psoriatic patients showed intermediate levels (12.3±21.6%). We conclude that psoriasis is associated with substantial alteration in the composition and representation of the cutaneous bacterial biota.Keywords
This publication has 43 references indexed in Scilit:
- Psoriasis is associated with increased β-defensin genomic copy numberNature Genetics, 2007
- The Human Microbiome ProjectNature, 2007
- Heart disease in psoriasisJournal of the American Academy of Dermatology, 2007
- Molecular analysis of human forearm superficial skin bacterial biotaProceedings of the National Academy of Sciences, 2007
- Molecular Analysis of Fungal Microbiota in Samples from Healthy Human Skin and Psoriatic LesionsJournal of Clinical Microbiology, 2006
- NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genesNucleic Acids Research, 2006
- Metagenomic Analysis of the Human Distal Gut MicrobiomeScience, 2006
- Staphylococcal toxins in patients with psoriasis, atopic dermatitis, and erythroderma, and in healthy control subjectsJournal of the American Academy of Dermatology, 2005
- Incidental detection of S. pyogenes-DNA in psoriatic skin by PCRArchiv für dermatologische Forschung, 2005
- From dissimilarities among species to dissimilarities among communities: a double principal coordinate analysisJournal of Theoretical Biology, 2004