16S Ribosomal RNA Methylation: Emerging Resistance Mechanism against Aminoglycosides
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Open Access
- 1 July 2007
- journal article
- Published by Oxford University Press (OUP) in Clinical Infectious Diseases
- Vol. 45 (1), 88-94
- https://doi.org/10.1086/518605
Abstract
Methylation of 16S ribosomal RNA (rRNA) has recently emerged as a new mechanism of resistance against aminoglycosides among gram-negative pathogens belonging to the family Enterobacteriaceae and glucose-nonfermentative microbes, including Pseudomonas aeruginosa and Acinetobacter species. This event is mediated by a newly recognized group of 16S rRNA methylases, which share modest similarity to those produced by aminoglycoside-producing actinomycetes. Their presence confers a high level of resistance to all parenterally administered aminoglycosides that are currently in clinical use. The responsible genes are mostly located on transposons within transferable plasmids, which provides them with the potential to spread horizontally and may in part explain the already worldwide distribution of this novel resistance mechanism. Some of these organisms have been found to coproduce extended-spectrum β-lactamases or metallo-β-lactamases, contributing to their multidrug-resistant phenotypes. A 2-tiered approach, consisting of disk diffusion tests followed by confirmation with polymerase chain reaction, is recommended for detection of 16S rRNA methylase–mediated resistance.Keywords
This publication has 23 references indexed in Scilit:
- The bacterial ribosome as a target for antibioticsNature Reviews Microbiology, 2005
- Molecular Insights into Aminoglycoside Action and ResistanceChemical Reviews, 2004
- Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical IsolatesAntimicrobial Agents and Chemotherapy, 2004
- Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosaThe Lancet, 2003
- Plasmid-Mediated High-Level Resistance to Aminoglycosides in Enterobacteriaceae Due to 16S rRNA MethylationAntimicrobial Agents and Chemotherapy, 2003
- Aminoglycosides: Perspectives on Mechanisms of Action and Resistance and Strategies to Counter ResistanceAntimicrobial Agents and Chemotherapy, 2000
- Decoding fidelity at the ribosomal A and P sites: influence of mutations in three different regions of the decoding domain in 16S rRNANucleic Acids Research, 1997
- HOW ANTIBIOTIC-PRODUCING ORGANISMS AVOID SUICIDEAnnual Review of Microbiology, 1989
- Sites of action of two ribosomal RNA methylases responsible for resistance to aminoglycosidesJournal of Molecular Biology, 1987
- Increased translational fidelity caused by the antibiotic kasugamycin and ribosomal ambiguity in mutants harbouring the ksgA geneFEBS Letters, 1984