Rice Seed Priming with Picomolar Rutin Enhances Rhizospheric Bacillus subtilis CIM Colonization and Plant Growth
Open Access
- 7 January 2016
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 11 (1), e0146013
- https://doi.org/10.1371/journal.pone.0146013
Abstract
The effect of rutin, a bioflavonoid on the growth and biofilm formation of Bacillus subtilis strain CIM was investigated. In addition to swimming, swarming, and twitching potentials of B. subtilis CIM (BS), one picomolar (1 pM) of rutin was also observed to boost the biofilm forming ability of the bacterium. Bio-priming of rice seeds with BS and rutin not only augmented root and shoot lengths but also the photosynthetic pigments like chlorophyll and carotenoid. Similarly, high accumulation of phenolic and flavonoid contents was observed in the leaves. Fluorescent microscopic images revealed that BS plus rutin enhanced callose deposition in the leaves. It was also established that the least formation of reactive oxygen species in BS plus rutin treated rice plants was due to higher free radicals scavenging activity and total antioxidant potential. The results highlight chemo attractant nature of BS towards rutin, which by enhancing biofilm formation and root colonization indirectly strengthened the plants’ defensive state.Keywords
This publication has 32 references indexed in Scilit:
- Production of reactive oxygen species and wound-induced resistance in Arabidopsis thaliana against Botrytis cinereaare preceded and depend on a burst of calciumBMC Plant Biology, 2013
- Soil bacteria augment Arabidopsis photosynthesis by decreasing glucose sensing and abscisic acid levels in plantaThe Plant Journal, 2008
- Root-Secreted Malic Acid Recruits Beneficial Soil BacteriaPlant Physiology, 2008
- Characteristics of Biofilm Formation byStreptococcus mutansin the Presence of SalivaInfection and Immunity, 2008
- Flavonoids induce germination of basidiospores of the ectomycorrhizal fungus Suillus bovinusMycorrhiza, 2007
- Bacterial Motility on a Surface: Many Ways to a Common GoalAnnual Review of Microbiology, 2003
- Priming in plant–pathogen interactionsTrends in Plant Science, 2002
- Rutin, the phenolglycoside from eucalyptus root exudates, stimulates Pisolithus hyphal growth at picomolar concentrationsNew Phytologist, 2001
- The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicalsFood Chemistry, 1999
- Correlation of defense gene induction defects with powdery mildew susceptibility inArabidopsisenhanced disease susceptibility mutantsThe Plant Journal, 1998