Physicochemical and biological properties of new tricalcium silicate‐based repair material doped with fluoride ions and zirconium oxide as radiopacifier
- 22 November 2021
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part B: Applied Biomaterials
- Vol. 110 (4), 862-870
- https://doi.org/10.1002/jbm.b.34966
Abstract
This study evaluated the physicochemical and biological properties of novel reparative materials composed of pure tricalcium silicate (Ca3SiO5), Ca3SiO5 doped with fluoride ions (Ca3SiO5-F) and their association with ZrO2 (Ca3SiO5 + ZrO2, Ca3SiO5-F + ZrO2), in comparison with Biodentine (BIO). Setting time radiopacity, pH, solubility, and dimensional change were evaluated based on ISO 6876 Standard. Volumetric change and flow/filling were assessed by microcomputed tomography (micro-CT). Biological properties were evaluated by the MTT assay 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), Neutral Red (NR), cell migration, alkaline phosphatase activity (ALP), and Alizarin Red Staining (ARS) assays. Statistical analysis was performed by ANOVA, Tukey, or Bonferroni tests (α = .05). Ca3SiO5-F + ZrO2 had higher radiopacity, shorter setting time, and lower solubility and volumetric loss than BIO (p < .05). Ca3SiO5-F + ZrO2 had flow and filling capacity similar to BIO (p > .05). All the cements evaluated had an alkaline pH. Ca3SiO5-F + ZrO2 demonstrated cell viability similar to negative control (p > .05), increase in ALP activity in 7 days, mineralized nodule production in 21 days and repair capacity according to cell migration. In conclusion, Ca3SiO5-F + ZrO2 had adequate setting time, radiopacity, solubility, and dimensional change. This material presented low volumetric change besides flow and filling capacity in micro-CT assessment. In addition, Ca3SiO5-F + ZrO2 was biocompatible and bioactive, suggesting its use as reparative material.This publication has 35 references indexed in Scilit:
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