Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours
Open Access
- 15 November 2012
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 2 (1), 865
- https://doi.org/10.1038/srep00865
Abstract
Correlative light and electron microscopy promises to combine molecular specificity with nanoscale imaging resolution. However, there are substantial technical challenges including reliable co-registration of optical and electron images, and rapid optical signal degradation under electron beam irradiation. Here, we introduce a new approach to solve these problems: imaging of stable optical cathodoluminescence emitted in a scanning electron microscope by nanoparticles with controllable surface chemistry. We demonstrate well-correlated cathodoluminescence and secondary electron images using three species of semiconductor nanoparticles that contain defects providing stable, spectrally-distinguishable cathodoluminescence. We also demonstrate reliable surface functionalization of the particles. The results pave the way for the use of such nanoparticles for targeted labeling of surfaces to provide nanoscale mapping of molecular composition, indicated by cathodoluminescence colour, simultaneously acquired with structural electron images in a single instrument.Keywords
This publication has 40 references indexed in Scilit:
- Spectrally and spatially resolved cathodoluminescence of nanodiamonds: local variations of the NV0emission propertiesNanotechnology, 2012
- Wiring specificity in the direction-selectivity circuit of the retinaNature, 2011
- Network anatomy and in vivo physiology of visual cortical neuronsNature, 2011
- Single-Synapse Analysis of a Diverse Synapse Population: Proteomic Imaging Methods and MarkersNeuron, 2010
- Synthesis and luminescent properties of Ce3+ doped LuAG nano-sized powders by mixed solvo-thermal methodJournal of Rare Earths, 2010
- Super-Resolution Fluorescence MicroscopyAnnual Review of Biochemistry, 2009
- Following the wiresNature Methods, 2007
- Characterization and application of single fluorescent nanodiamonds as cellular biomarkersProceedings of the National Academy of Sciences, 2007
- Cathodoluminescence investigation of organic materialsMicroscopy, 2005
- Scanning Electron Microscopy: Physics of Image Formation and Microanalysis, Second EditionMeasurement Science and Technology, 2000