Liquid Chromatography-Tandem Mass Spectrometry – Application in the Clinical Laboratory
- 21 January 2003
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 41 (2), 117-26
- https://doi.org/10.1515/cclm.2003.020
Abstract
This review provides a concise survey of liquid chromatography tandem mass spectrometry (LCTMS) as an emerging technology in clinical chemistry. The combination of two mass spectrometers with an interposed collision cell characterizes LCTMS as an analytical technology on its own and not just as a more specific detector for HPLC compared with conventional techniques. In LCTMS, liquid chromatography is rather used for sample preparation but not for complete resolution of compounds of interest. The instrument technology of LCTMS is complex and comparatively expensive; however, in routine use, methods are far more rugged compared to conventional chromatographic techniques and enable highthroughput analyses with very limited manual handling steps. Moreover, compared to both gas chromatographymass spectrometry (GCMS) and conventional HPLC techniques, LCTMS is substantially more versatile with respect to the spectrum of analyzable compounds. For these reasons it is likely that LCTMS will gain far more widespread use in the clinical laboratory than HPLC and GCMS ever did. In this article, the key features of LCTMS are described, method development is explained, typical fields of application are discussed, and personal experiences are related.Keywords
This publication has 14 references indexed in Scilit:
- Application of tandem mass spectrometry to biochemical genetics and newborn screeningClinica Chimica Acta; International Journal of Clinical Chemistry, 2002
- Quantification of Sirolimus by Liquid Chromatography-Tandem Mass Spectrometry Using On-Line Solid-Phase Extractioncclm, 2002
- Validation of the Phenylalanine/Tyrosine Ratio Determined by Tandem Mass Spectrometry: Sensitive Newborn Screening for Phenylketonuriacclm, 2002
- Potential Lack of Specificity Using Electrospray Tandem-Mass Spectrometry for the Analysis of Mycophenolic Acid in SerumTherapeutic Drug Monitoring, 2001
- Clinical applications of tandem mass spectrometry: ten years of diagnosis and screening for inherited metabolic diseasesJournal of Chromatography B: Biomedical Sciences and Applications, 2001
- Quantitative Analysis by Liquid Chromatography–Tandem Mass Spectrometry of Deuterium-Labeled and Unlabeled Vitamin E in Biological SamplesAnalytical Biochemistry, 2001
- Determination of Serum Cortisol by Isotope-Dilution Liquid-Chromatography Electrospray Ionization Tandem Mass Spectrometry with On-line Extractioncclm, 2001
- High-throughput quantitative bioanalysis by LC/MS/MSBiomedical Chromatography, 2000
- Exploring the boundaries of bioanalytical quantitative LC–MS–MSTrAC Trends in Analytical Chemistry, 2000
- Quantitative analysis of sirolimus (Rapamycin) in blood by high-performance liquid chromatography–electrospray tandem mass spectrometryJournal of Chromatography B: Biomedical Sciences and Applications, 1998