High-throughput quantitative bioanalysis by LC/MS/MS
- 18 September 2000
- journal article
- review article
- Published by Wiley in Biomedical Chromatography
- Vol. 14 (6), 422-429
- https://doi.org/10.1002/1099-0801(200010)14:6<422::aid-bmc25>3.0.co;2-i
Abstract
This review article discusses the most recent significant advances in the sample preparation and mass spectrometry aspects of high‐throughput bioanalysis by LC/MS/MS for the quantitation of drugs, metabolites and endogenous biomolecules in biological matrices. The introduction and implementation of automated 96‐well extraction has brought about high‐throughput approaches to the biological sample preparation techniques of solid‐phase extraction, liquid‐liquid extraction and protein precipitation. The fast‐flow on‐line extraction technique is a different high‐throughput approach that has also significantly speeded up analysis by LC/MS/MS. The use of pierceable caps for biological tubes further enhances the analysis speed and improves the safety in handling biological samples. The need for adequate chromatographic separation in order to eliminate interferences due to metabolites and/or matrix effects in LC/MS/MS is discussed. To highlight our limited understanding of atmospheric pressure ionization mass spectrometry, results from recent investigations that appear to be counter‐intuitive are presented. Looking ahead to the future, multiplexed LC/MS/MS systems and capillary LC are presented as areas that can bring about further improvements in analysis speed and sensitivity to quantitative bioanalysis by LC/MS/MS. Copyright © 2000 John Wiley & Sons, Ltd. Abbreviations used: API atmospheric pressure ionization LLE liquid–liquid extraction SPE solid‐phase extraction SRM selective reaction monitoring TOF time‐of‐flightKeywords
This publication has 48 references indexed in Scilit:
- Automated protein precipitation by filtration in the 96-well formatJournal of Chromatography B: Biomedical Sciences and Applications, 1999
- A new approach to the effective preparation of plasma samples for rapid drug quantitation using on-line solid phase extraction mass spectrometryRapid Communications in Mass Spectrometry, 1999
- Ultra-high flow rate capillary liquid chromatography with mass spectrometric detection for the direct analysis of pharmaceuticals in plasma at sub-nanogram per millilitre concentrationsRapid Communications in Mass Spectrometry, 1999
- The effects of sample preparation methods on the variability of the electrospray ionization response for model drug compoundsRapid Communications in Mass Spectrometry, 1999
- High throughput liquid chromatography/mass spectrometric analyses using a novel multiplexed electrospray interfaceRapid Communications in Mass Spectrometry, 1999
- Comparison of conventional, narrow-bore and capillary liquid chromatography/mass spectrometry for electrospray ionization mass spectrometry: practical considerationsJournal of Mass Spectrometry, 1999
- Optimisation and routine use of generic ultra-high flow-rate liquid chromatography with mass spectrometric detection for the direct on-line analysis of pharmaceuticals in plasmaJournal of Chromatography A, 1998
- The use of turbulent flow chromatography/mass spectrometry for the rapid, direct analysis of a novel pharmaceutical compound in plasmaRapid Communications in Mass Spectrometry, 1997
- Quantitation of SR 27417 in human plasma using electrospray liquid chromatography-tandem mass spectrometry: A study of ion suppressionJournal of the American Society for Mass Spectrometry, 1996
- The Use of Automated Solid Phase Extraction in the '96 well' Format for High Throughput Bioanalysis using Liquid Chromatography Coupled to Tandem Mass SpectrometryRapid Communications in Mass Spectrometry, 1996