Identification of Two Polysaccharides from Prunella vulgaris L. and Evaluation on Their Anti-Lung Adenocarcinoma Activity
Open Access
- 27 July 2010
- Vol. 15 (8), 5093-5103
- https://doi.org/10.3390/molecules15085093
Abstract
Prunella vulgaris L. (PV) has been used for tumor therapy in Traditional Chinese Medicine for centuries, however, systematic research on extracted PV polysaccharides believed to possess various biological activities, as well as their preventive and anti-tumor effects on lung cancer has not been reported. In this study, two polysaccharides (P31 and P32) were isolated from the aqueous extract of PV and purified through ethanol precipitation, followed by deproteination using DEAE-52 gel-filtration chromatography. The main monosaccharide composition of polysaccharide P32 was analyzed by GC. It was found that polysaccharide P32 consisted of rhamnose, arabinose, xylose, mannose, glucose and galactose in a molar ratio of 3.46:49.32:58.91:0.43:2.64: 3.11, respectively. In order to evaluate polysaccharide P32’s anti-lung adenocarcinoma activities and immunomodulation effects, a C57BL/6 mouse-Lewis lung carcinoma (LLC) model was established and investigated. Our results showed that polysaccharides of PV had anti-lung cancer activity and could increase the thymus index and the spleen index in tumor-bearing mice, suggesting possible immunomodulation effects.This publication has 14 references indexed in Scilit:
- Paclitaxel Efficacy is Increased by Parthenolide via Nuclear Factor- KappaB Pathways in In Vitro and In Vivo Human Non – Small Cell Lung Cancer ModelsCurrent Cancer Drug Targets, 2010
- Paclitaxel Efficacy is Increased by Parthenolide via Nuclear Factor- KappaB Pathways in In Vitro and In Vivo Human Non-Small Cell Lung Cancer ModelsCurrent Cancer Drug Targets, 2010
- Suppression of PMA-induced tumor cell invasion and metastasis by aqueous extract isolated from Prunella vulgaris via the inhibition of NF-κB-dependent MMP-9 expressionFood and Chemical Toxicology, 2010
- Analysis of the monosaccharide composition of purified polysaccharides in Ganoderma atrum by capillary gas chromatographyPhytochemical Analysis, 2009
- Immunostimulatory activity of aqueous extract isolated from Prunella vulgarisFood and Chemical Toxicology, 2009
- Molecular Weight and Monosaccharide Composition of Astragalus PolysaccharidesMolecules, 2008
- Effects of two Prunella species on lymphocyte proliferation and nitric oxide productionPhytotherapy Research, 2006
- Immune modulatory effects of Prunella vulgaris L.International Journal of Molecular Medicine, 2005
- Biological activities of Prunella vulgaris extractPhytotherapy Research, 2003
- Isolation, purification, and partial characterization of prunellin, an anti-HIV component from aqueous extracts of Prunella vulgarisAntiviral Research, 1989