Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides
Open Access
- 30 September 2008
- Vol. 13 (10), 2408-2415
- https://doi.org/10.3390/molecules13102408
Abstract
Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLC-GPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH2 column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1:3.45 and 1:6.25:17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.Keywords
This publication has 6 references indexed in Scilit:
- Merit of Astragalus polysaccharide in the improvement of early diabetic nephropathy with an effect on mRNA expressions of NF-κB and IκB in renal cortex of streptozotoxin-induced diabetic ratsJournal of Ethnopharmacology, 2007
- In vitro and in vivo anti-tumor effects of Astragalus membranaceusCancer Letters, 2007
- Preparation and suppressive effect of astragalus polysaccharide in glomerulonephritis ratsInternational Immunopharmacology, 2007
- Carbohydrate analysis by a phenol–sulfuric acid method in microplate formatAnalytical Biochemistry, 2005
- A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herbBiochemical and Biophysical Research Communications, 2004
- Isolation and characterization of plant cell walls and cell wall componentsMethods in enzymology, 1986