Estrogen Inhibits Interleukin-lβ-Induced Interleukin-6 Production by Human Osteoblast-like Cells
- 1 July 1998
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Interferon & Cytokine Research
- Vol. 18 (7), 479-483
- https://doi.org/10.1089/jir.1998.18.479
Abstract
Estrogen supplements are the primary pharmacologic intervention therapy to prevent and treat loss of bone mass (osteoporosis) in postmenopausal women. Furthermore, at sites of local inflammation near bone, estrogen-deficient women are significantly more susceptible to bone loss than are estrogen-sufficient women. In the present study, we investigate whether estrogen modulates osteoblast (MG-63) production of interleukin6 (IL-6), an osteoclast recruitment and differentiation of cytokine, in the presence of the proinflammatory cytokine, IL-1β. Using enzyme-linked immunosorbent assay (ELISA), we demonstrate that II-1β significantly enhances IL-6 secretion into culture supernatants in a dose-dependent and time-dependent manner. Using reverse-transcriptase polymerase chain reaction (RT-PCR) and ELISA respectively, we demonstrate further that levels of 17β-estradiol (active metabolite of estrogen) ≥ those found in serum of estrogen-sufficient women inhibit steady-state IL-6 mRNA levels as well as inhibit secretion of IL-6 into culture supernatants. One mechanism by which estrogen therapy preserves bone mass in areas of inflammation may be via inhibition of IL1β-stimulated obsteoblast-derived IL-6.Keywords
This publication has 34 references indexed in Scilit:
- Differential Ligand Activation of Estrogen Receptors ERα and ERβ at AP1 SitesScience, 1997
- Serum N-terminal osteocalcin is a good indicator for estimating responders to hormone replacement therapy in postmenopausal womenJournal of Bone and Mineral Research, 1996
- Cytokine Expression in Multiple Myeloma and Monoclonal Gammopathy: Analysis by Reverse Transcription/Polymerase Chain Reaction and Quantitative PCRLeukemia & Lymphoma, 1996
- New insights into the cellular, biochemical, and molecular basis of postmenopausal and senile osteoporosis: Roles of IL-6 and gp130International Journal of Immunopharmacology, 1995
- Correlates of bone mineral density in the postmenopausal estrogen/progestin interventions trialJournal of Bone and Mineral Research, 1994
- Increased Osteoclast Development After Estrogen Loss: Mediation by Interleukin-6Science, 1992
- Osteoclast resorption — stimulating activity is associated with the osteoblast cell surface and/or the extracellular matrixBiochemical and Biophysical Research Communications, 1991
- Parathyroid hormone (PTH) and PTH-like protein (PLP) stimulate interleukin-6 production by osteogenic cells: A possible role of interleukin-6 in osteoclastogenesisBiochemical and Biophysical Research Communications, 1989
- Hip Fracture and the Use of Estrogens in Postmenopausal WomenNew England Journal of Medicine, 1987
- Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform ExtractionAnalytical Biochemistry, 1987