A phosphorus NMR study of the reaction products of cis‐diamminedichloroplatinum (II) with a double‐helical oligonucleotide and with DNA
Open Access
- 29 October 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 176 (2), 393-397
- https://doi.org/10.1016/0014-5793(84)81204-1
Abstract
The structural distortion of oligonucleotides upon cis‐PtCl2(NH3)2{d(T‐C‐T‐C‐G‐G‐T‐C‐T'‐C‐N7(5) N7(6)} reveals shifting of 4 phosphorus resonances due to platination. 3 Resonances could be assigned by selective 31P‐irradiation, showing P(6) (P between the two Gs) to be shifted 1.5 ppm to low field. In the concomitant double strands P(6) is shifted 0.9 ppm to lower field. A similar peak has been observed in platinated salmon sperm DNA (37°C), indicating that Pt‐binding to GpG‐fragments in DNA is similar to that found for the decanucleotide, so the distortion of DNA might be comparable.Keywords
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