Evidence for similar structural changes on binding of platinum anti‐tumor agents to DNA and nucleosomes
- 29 October 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 176 (2), 389-392
- https://doi.org/10.1016/0014-5793(84)81203-x
Abstract
The influence of Pt(II) compounds on the 31P NMR spectra of natural DNA, synthetic polynucleotides, and nucleosomes was investigated. With Pt complexes which are anti-tumor agents, a new peak or shoulder centered at ~ 1.2 ppm downfield from the untreated DNA signal was observed. When Pt compounds known not to be anti-tumor agents were studied, no such new signal was observed. The most reasonable explanation for the downfield resonance is that it is a consequence of a structural change induced in the DNA by the anti-tumor agent. Since the effect of the Pt compounds on nucleosomes was similar, the same structural change is probably occurring in DNA in solution and in nucleosomes. A nonalternating dG · dC polymer, but not alternating G·C or any A·T polymers, exhibited a similar spectral change and this finding suggests that the structural change in the DNA arises primarily from reaction of Pt anti-tumor agents with adjacent G residuesKeywords
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