Characterization of White Spot Syndrome Virus Envelope Protein VP51A and Its Interaction with Viral Tegument Protein VP26
Open Access
- 15 December 2008
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 82 (24), 12555-12564
- https://doi.org/10.1128/jvi.01238-08
Abstract
In this study, we characterize a novel white spot syndrome virus (WSSV) structural protein, VP51A (WSSV-TW open reading frame 294), identified from a previous mass spectrometry study. Temporal-transcription analysis showed that vp51A is expressed in the late stage of WSSV infection. Gene structure analysis showed that the transcription initiation site of vp51A was 135 bp upstream of the translation start codon. The poly(A) addition signal overlapped with the translation stop codon, TAA, and the poly(A) tail was 23 bp downstream of the TAA. Western blot analysis of viral protein fractions and immunoelectron microscopy both suggested that VP51A is a viral envelope protein. Western blotting of the total proteins extracted from WSSV virions detected a band that was close to the predicted 51-kDa mass, but the strongest signal was around 72 kDa. We concluded that this 72-kDa band was in fact the full-length VP51A protein. Membrane topology assays demonstrated that the VP51A 72-kDa protein is a type II transmembrane protein with a highly hydrophobic transmembrane domain on its N terminus and a C terminus that is exposed on the surface of the virion. Coimmunoprecipitation, colocalization, and yeast two-hybrid assays revealed that VP51A associated directly with VP26 and indirectly with VP28, with VP26 acting as a linker protein in the formation of a VP51A-VP26-VP28 complex.Keywords
This publication has 47 references indexed in Scilit:
- Crystal Structures of Major Envelope Proteins VP26 and VP28 from White Spot Syndrome Virus Shed Light on Their Evolutionary RelationshipJournal of Virology, 2007
- White Spot Syndrome Virus Annexes a Shrimp STAT To Enhance Expression of the Immediate-Early Gene ie1Journal of Virology, 2007
- Proteomic Analysis of the Major Envelope and Nucleocapsid Proteins of White Spot Syndrome VirusJournal of Virology, 2006
- White spot syndrome virus VP24 interacts with VP28 and is involved in virus infectionJournal of General Virology, 2006
- Multiple envelope proteins are involved in white spot syndrome virus (WSSV) infection in crayfishArchiv für die gesamte Virusforschung, 2006
- Double-Stranded RNA Induces Sequence-Specific Antiviral Silencing in Addition to Nonspecific Immunity in a Marine Shrimp: Convergence of RNAInterference and Innate Immunity in the Invertebrate Antiviral Response?Journal of Virology, 2005
- Budding events in herpesvirus morphogenesisVirus Research, 2004
- Genomic and Proteomic Analysis of Thirty-Nine Structural Proteins of Shrimp White Spot Syndrome VirusJournal of Virology, 2004
- Predicting transmembrane protein topology with a hidden markov model: application to complete genomesJournal of Molecular Biology, 2001
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982