Crystal Structures of Major Envelope Proteins VP26 and VP28 from White Spot Syndrome Virus Shed Light on Their Evolutionary Relationship
- 15 June 2007
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 81 (12), 6709-6717
- https://doi.org/10.1128/jvi.02505-06
Abstract
White spot syndrome virus (WSSV) is a virulent pathogen known to infect various crustaceans. It has bacilliform morphology with a tail-like appendage at one end. The envelope consists of four major proteins. Envelope structural proteins play a crucial role in viral infection and are believed to be the first molecules to interact with the host. Here, we report the localization and crystal structure of major envelope proteins VP26 and VP28 from WSSV at resolutions of 2.2 and 2.0 Å, respectively. These two proteins alone account for approximately 60% of the envelope, and their structures represent the first two structural envelope proteins of WSSV. Structural comparisons among VP26, VP28, and other viral proteins reveal an evolutionary relationship between WSSV envelope proteins and structural proteins from other viruses. Both proteins adopt β-barrel architecture with a protruding N-terminal region. We have investigated the localization of VP26 and VP28 using immunoelectron microscopy. This study suggests that VP26 and VP28 are located on the outer surface of the virus and are observed as a surface protrusion in the WSSV envelope, and this is the first convincing observation for VP26. Based on our studies combined with the literature, we speculate that the predicted N-terminal transmembrane region of VP26 and VP28 may anchor on the viral envelope membrane, making the core β-barrel protrude outside the envelope, possibly to interact with the host receptor or to fuse with the host cell membrane for effective transfer of the viral infection. Furthermore, it is tempting to extend this host interaction mode to other structural viral proteins of similar structures. Our finding has the potential to extend further toward drug and vaccine development against WSSV.Keywords
This publication has 48 references indexed in Scilit:
- Proteomic Analysis of the Major Envelope and Nucleocapsid Proteins of White Spot Syndrome VirusJournal of Virology, 2006
- Identification of a Novel Nonstructural Protein, VP9, from White Spot Syndrome Virus: Its Structure Reveals a Ferredoxin Fold with Specific Metal Binding SitesJournal of Virology, 2006
- PmRab7 Is a VP28-Binding Protein Involved in White Spot Syndrome Virus Infection inShrimpJournal of Virology, 2006
- Viral membrane fusion: is glycoprotein G of rhabdoviruses a representative of a new class of viral fusion proteins?Brazilian Journal of Medical and Biological Research, 2005
- Genomic and Proteomic Analysis of Thirty-Nine Structural Proteins of Shrimp White Spot Syndrome VirusJournal of Virology, 2004
- Conformational change and protein–protein interactions of the fusion protein of Semliki Forest virusNature, 2004
- ESPript: analysis of multiple sequence alignments in PostScript.Bioinformatics, 1999
- [20] Processing of X-ray diffraction data collected in oscillation modeMethods in Enzymology, 1997
- The structure of simian virus 40 refined at 3.1 å resolutionStructure, 1996
- Main-chain Bond Lengths and Bond Angles in Protein StructuresJournal of Molecular Biology, 1993