Potent Anti-Inflammatory and Antiadipogenic Properties of Bamboo (Sasa coreana Nakai) Leaves Extract and Its Major Constituent Flavonoids
- 27 July 2017
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 65 (31), 6665-6673
- https://doi.org/10.1021/acs.jafc.7b02203
Abstract
The pro-inflammatory response and recruitment of macrophages into adipose tissue contribute to metabolic dysfunction. Here, we reported the anti-inflammatory and antiadipogenic effects of the methanol (MeOH) extract and ethyl acetate (EtOAc) fraction of bamboo leaf and its molecular mechanism in RAW264.7 cells and 3T3-L1 adipocytes, respectively. Functional macrophage migration assays also were performed. Surprisingly, the EtOAc fraction of MeOH extracts from native Korean plant species Sasa coreana Nakai (SCN) has shown potent anti-inflammatory properties; SCN pretreatment inhibited nitric oxide (NO) production (p < 0.01) and inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated macrophages. Inflammatory genes induced by LPS, including TNFα, IL-1β, and IL-6, were significantly attenuated by SCN (p < 0.01). Pretreatment with SCN antagonized NF-κB nuclear translocation and the simultaneous degradation of inhibitory κB protein. Furthermore, SCN selectively inhibited the LPS-induced phosphorylation of JNK (p < 0.01) and p38 (p < 0.05) but not ERK (p > 0.05). Similar to leaf extracts of other bamboo species, we identified that SCN contained several flavonoids including orientin, isoorientin, and vitexin; these compounds inhibited LPS-induced NO production (p < 0.05) and iNOS expression. In addition, SCN inhibited adipocyte differentiation in a dose-dependent manner, as demonstrated by Oil Red O staining and the protein expression of mature adipogenic marker genes. Treatment with the major flavonoids of SCN also inhibited adipogenesis. Furthermore, conditioned medium obtained from adipocytes stimulated macrophage chemotaxis, whereas medium from adipocytes treated with SCN significantly inhibited macrophage migration. Therefore, SCN is a potential therapeutic agent for the prevention of inflammation and obesity.Funding Information
- National Research Foundation of Korea (2015H1C1A1035883, R13-2008-010-00000-0)
This publication has 35 references indexed in Scilit:
- Increased Macrophage Migration Into Adipose Tissue in Obese MiceDiabetes, 2012
- Rapid Screening for FlavoneC-Glycosides in the Leaves of Different Species of Bamboo and Simultaneous Quantitation of Four Marker Compounds by HPLC-UV/DADInternational Journal of Analytical Chemistry, 2012
- PPARs are a unique set of fatty acid regulated transcription factors controlling both lipid metabolism and inflammationBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2011
- Adipokines in inflammation and metabolic diseaseNature Reviews Immunology, 2011
- Rise and fall of anti-obesity drugsWorld Journal of Diabetes, 2011
- Transcriptional factors that promote formation of white adipose tissueMolecular and Cellular Endocrinology, 2010
- Immunostimulation-Mediated Anti-Tumor Activity of Bamboo (Sasa senanensis) Leaf Extracts Obtained under ‘Vigorous’ ConditionEvidence-Based Complementary and Alternative Medicine, 2010
- Macrophage infiltration into adipose tissue: initiation, propagation and remodelingFuture Lipidology, 2008
- Obesity induces a phenotypic switch in adipose tissue macrophage polarizationJCI Insight, 2007
- Obesity and insulin resistanceJCI Insight, 2000