Stability of DNA Origami Nanoarrays in Cell Lysate
Top Cited Papers
- 2 March 2011
- journal article
- letter
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 11 (4), 1477-1482
- https://doi.org/10.1021/nl1040836
Abstract
Scaffolded DNA origami, a method to create self-assembled nanostructures with spatially addressable features, has recently been used to develop water-soluble molecular chips for label-free RNA detection, platforms for deterministic protein positioning, and single molecule reaction observatories. These applications highlight the possibility of exploiting the unique properties and biocompatibility of DNA nanostructures in live, cellular systems. Herein, we assembled several DNA origami nanostructures of differing shape, size and probes, and investigated their interaction with lysate obtained from various normal and cancerous cell lines. We separated and analyzed the origami−lysate mixtures using agarose gel electrophoresis and recovered the DNA structures for functional assay and subsequent microscopic examination. Our results demonstrate that DNA origami nanostructures are stable in cell lysate and can be easily separated from lysate mixtures, in contrast to natural, single- and double-stranded DNA. Atomic force microscope (AFM) and transmission electron microscope (TEM) images show that the DNA origami structures are fully intact after separation from cell lysates and hybridize to their targets, verifying the superior structural integrity and functionality of self-assembled DNA origami nanostructures relative to conventional oligonucleotides. The stability and functionality of DNA origami structures in cell lysate validate their use for biological applications, for example, as programmable molecular rafts or disease detection platforms.Keywords
This publication has 34 references indexed in Scilit:
- The Label-Free Unambiguous Detection and Symbolic Display of Single Nucleotide Polymorphisms on DNA OrigamiNano Letters, 2011
- Molecular robots guided by prescriptive landscapesNature, 2010
- Multilayer DNA Origami Packed on a Square LatticeJournal of the American Chemical Society, 2009
- Folding DNA into Twisted and Curved Nanoscale ShapesScience, 2009
- Self-assembly of DNA into nanoscale three-dimensional shapesNature, 2009
- Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of bloodNature Biotechnology, 2008
- Fabrication of Microfluidic Reactors and Mixing Studies for Luciferase DetectionAnalytical Chemistry, 2008
- Self-assembled DNA nanostructures for distance-dependent multivalent ligand–protein bindingNature Nanotechnology, 2008
- Continuous flow microfluidic device for cell separation, cell lysis and DNA purificationAnalytica Chimica Acta, 2007
- Microchip‐Based Cell Lysis and DNA Extraction from Sperm Cells for Application to Forensic AnalysisJournal of Forensic Sciences, 2006