The synthesis of human α‐2‐HS glycoprotein is down‐regulated by cytokines in hepatoma HepG2 cells
- 5 December 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 241 (1-2), 191-194
- https://doi.org/10.1016/0014-5793(88)81059-7
Abstract
The regulation of the synthesis of α-2-HS glycoprotein (AHSG) by inflammatory mediators from activated monocytes was studied on the human hepatoma cell line HepG2 and compared to that of albumin. Monocyte-conditioned medium, recombinant human interleukin-6 (rhIL6) and interleukin-1β(rhIL1β) all down-regulated the synthesis of AHSG. This decrease was found both at the protein and the mRNA level. The most efficient mediator was the monocyte-conditioned medium, when rhIL1β was found to be less efficient than rhIL6. The combination of rhIL6 and rhIL1β resulted in an additive down-regulation of the AHSG mRNA levels. Similar results were obtained with albumin. These data indicate that AHSG is a negative acute-phase protein whose synthesis is regulated by cytokines in a manner similar to that of albumin.Keywords
This publication has 20 references indexed in Scilit:
- Regulation of synthesis and secretion of major rat acute‐phase proteins by recombinant human interleukin‐6 (BSF‐2/1L‐6) in hepatocyte primary culturesEuropean Journal of Biochemistry, 1988
- Recombinant human B cell stimulatory factor 2 (BSF‐2/IFN‐β2) regulates β‐fibrinogen and albumin mRNA levels in Fao‐9 cellsFEBS Letters, 1987
- The inflammation mediators interleukin 1 and hepatocyte‐stimulating factor are differently regulated in human monocytesEuropean Journal of Immunology, 1987
- Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulinNature, 1986
- Monocyte-conditioned medium, interleukin-1, and tumor necrosis factor stimulate the acute phase response in human hepatoma cells in vitro.The Journal of cell biology, 1986
- Murine interleukin 1 stimulates α2‐macroglobulin synthesis in rat hepatocyte primary culturesFEBS Letters, 1985
- Interleukin-1 and the Pathogenesis of the Acute-Phase ResponseNew England Journal of Medicine, 1984
- Serum concentration of human alpha 2 HS glycoprotein during the inflammatory process: evidence that alpha 2 HS glycoprotein is a negative acute-phase reactant.JCI Insight, 1979
- Detection of a mediator derived from endotoxin-stimulated macrohpages that induces the acute phase serum amyloid A response in mice.The Journal of Experimental Medicine, 1979
- Opsonic Properties of Human Serum Alpha-2 HS GlycoproteinImmunological Communications, 1974