Isolation and characterization of the human pulmonary surfactant apoprotein gene
- 1 September 1985
- journal article
- letter
- Published by Springer Science and Business Media LLC in Nature
- Vol. 317 (6035), 361-363
- https://doi.org/10.1038/317361a0
Abstract
Pulmonary surfactant is a phospholipid–protein complex which serves to lower the surface tension at the air–liquid interface in the alveoli of the mammalian lung and is essential for normal respiration1. Inadequate levels of surfactant at birth, a frequent situation in premature infants, results in respiratory failure. In all species examined, surfactant is composed primarily of dipalmitoylphosphatidylcholine and two major protein species of relative molecular mass (Mr) 32,000 (32K) and 10K (refs 2–5). Reconstitution in vitro of purified 32K pulmonary surfactant apoprotein (PSAP) with synthetic lipids forms a lipoprotein complex that lowers surface tension by spreading to create a thin interfacial film6,7. Here we describe the cloning of the human PSAP gene and complementary DNA, and discuss features of the unusual encoded protein.Keywords
This publication has 26 references indexed in Scilit:
- An electrophoretic and immunochemical characterization of human surfactant-associated proteinsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1984
- Sequence-specific binding of glucocorticoid receptor to MTV DNA at sites within and upstream of the transcribed regionCell, 1983
- Respiratory Distress Syndrome – Update 1982Pediatric Clinics of North America, 1982
- Analysis of pulmonary surfactant apoproteins by electrophoresisBiochimica et Biophysica Acta (BBA) - Protein Structure, 1981
- Isolation of a major apolipoprotein of canine and murine pulmonary surfactant Biochemical and immunochemical characteristicsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoidsNature, 1981
- The ovalbumin gene - sequence of putative control regionsNucleic Acids Research, 1980
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Isolation of apoproteins from canine surface active materialAmerican Journal of Physiology-Legacy Content, 1973