Molecular cloning of unintegrated closed circular DNA of porcine retrovirus
- 31 March 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 198 (2), 339-343
- https://doi.org/10.1016/0014-5793(86)80432-x
Abstract
Viral DNA of unintegrated closed circular form was isolated from a swine kidney cell line (SKL) which was infected with a porcine retrovirus Tsukuba-1 (PRetV) produced from a swine malignant lymphoma-derived cell line. Shimozuma-1 and cloned using a lambda phage vector, Charon 21A. One of ten independent clones contained the 8.3 kb DNA fragment as an insert, which was thought to be a full length of viral DNA molecule carrying a long terminal repeat (LTR) sequence. We have analyzed this insert by mapping the recognition sites of some restriction endonucleases by Southern blot hybridization with appropriate probes.Keywords
This publication has 13 references indexed in Scilit:
- Complete nucleotide sequence of an infectious clone of human T-cell leukemia virus type II: an open reading frame for the protease gene.Proceedings of the National Academy of Sciences, 1985
- Some characteristics of a porcine retrovirus from a cell line derived from swine malignant lymphomasFEBS Letters, 1985
- Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA.Proceedings of the National Academy of Sciences, 1983
- Long terminal repeats of endogenous mouse mammary tumour virus contain a long open reading frame which extends into adjacent sequencesNature, 1982
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- Characterization of dna complementary to nucleotide sequences at the 5′-terminus of the avian sarcoma virus genomeVirology, 1977
- Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA CloningScience, 1977
- Efficient transcription of RNA into DNA by avian sarcoma virus polymeraseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Selective extraction of polyoma DNA from infected mouse cell culturesJournal of Molecular Biology, 1967