Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA Cloning
- 8 April 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4286), 161-169
- https://doi.org/10.1126/science.847462
Abstract
The Charon lambda bacteriophages have been developed as vectors for cloning. Their construction incorporates mutations that make them simple to use and also greatly increases their safety for the biological containment of cloned recombinant DNA. Three of the Charon vector phages, 3A, 4A, and 16A, have been certified for use as EK2 vector-host systems, when propagated in bulk in a special bacterial host, DP50SupF. We present here some of the data on which the safety of these systems was evaluated. DNA fragments ranging in size from 0 to 2.2 X 10(4) base pairs can be cloned in these EK2 Charon phages.This publication has 38 references indexed in Scilit:
- In vitro packaging of a λ Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1Gene, 1977
- Improved derivative of a phage λ EK2 vector for cloning recombinant DNANature, 1976
- Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coliJournal of Molecular Biology, 1975
- Studies on the cleavage of bacteriophage lambda DNA with EcoRI restriction endonucleaseJournal of Molecular Biology, 1975
- DNA packaging steps in bacteriophage lambda head assemblyJournal of Molecular Biology, 1975
- DNA methylases of Hemophilus influenzae Rd: I. Purification and propertiesJournal of Molecular Biology, 1973
- Deletion mutants of bacteriophage lambda: I. Isolation and initial characterizationJournal of Molecular Biology, 1971
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970
- Mapping of Deletions and Substitutions in Heteroduplex DNA Molecules of Bacteriophage Lambda by Electron MicroscopyScience, 1969
- The linear insertion of a prophage into the chromosome of E. Coli shown by deletion mappingBiochemical and Biophysical Research Communications, 1965