Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds
- 18 August 2010
- journal article
- research article
- Published by Wiley in Macromolecular Bioscience
- Vol. 10 (11), 1330-1337
- https://doi.org/10.1002/mabi.201000165
Abstract
Polymer scaffolds that direct elongation and orientation of cultured cells can enable tissue engineered muscle to act as a mechanically functional unit. We combined micromolding and microablation technologies to create muscle tissue engineering scaffolds from the biodegradable elastomer poly(glycerol sebacate). These scaffolds exhibited well defined surface patterns and pores and robust elastomeric tensile mechanical properties. Cultured C2C12 muscle cells penetrated the pores to form spatially controlled engineered tissues. Scanning electron and confocal microscopy revealed muscle cell orientation in a preferential direction, parallel to micromolded gratings and long axes of microablated anisotropic pores, with significant individual and interactive effects of gratings and pore design.Keywords
This publication has 48 references indexed in Scilit:
- Finite element analysis of an accordion-like honeycomb scaffold for cardiac tissue engineeringJournal of Biomechanics, 2010
- Electrospun Fibrous Scaffolds with Multiscale and Photopatterned PorosityMacromolecular Bioscience, 2010
- Perfusion seeding of channeled elastomeric scaffolds with myocytes and endothelial cells for cardiac tissue engineeringBiotechnology Progress, 2010
- Advanced Material Strategies for Tissue Engineering ScaffoldsAdvanced Materials, 2009
- Engineering Substrate Topography at the Micro‐ and Nanoscale to Control Cell FunctionAngewandte Chemie-International Edition, 2009
- Novel Micropatterned Cardiac Cell Cultures with Realistic Ventricular MicrostructureBiophysical Journal, 2009
- Engineered skeletal muscle tissue networks with controllable architectureBiomaterials, 2008
- Accordion-like honeycombs for tissue engineering of cardiac anisotropyNature Materials, 2008
- Effects of electrical stimulation in C2C12 muscle constructsJournal of Tissue Engineering and Regenerative Medicine, 2008
- Pre‐treatment of synthetic elastomeric scaffolds by cardiac fibroblasts improves engineered heart tissueJournal of Biomedical Materials Research Part A, 2007