Effects of electrical stimulation in C2C12 muscle constructs
- 30 May 2008
- journal article
- research article
- Published by Hindawi Limited in Journal of Tissue Engineering and Regenerative Medicine
- Vol. 2 (5), 279-287
- https://doi.org/10.1002/term.93
Abstract
Electrical stimulation affects the deposition of extracellular matrices and cellular differentiation. Type I collagen is one of the most abundant extracellular matrix proteins; however, not much is known about the effects of electrical stimulation on collagen type I deposition in C2C12 cells. Thus, we studied the effects of electrical voltage and stimulation frequency in 3D cultured C2C12 muscle cells in terms of metabolic activity, type I collagen deposition and cell morphology. Electrically excitable C2C12 muscle cells were seeded in collagen scaffolds and stimulated with rectangular signals of voltage (2, 5, 7 V) and frequency (1, 2 Hz), using parallel carbon electrodes spaced 1 cm apart. Metabolic activity was quantified by the glucose:lactate concentration ratio in the medium. Apoptotic activity was assessed by TUNEL staining and changes in collagen deposition were identified by immunohistology. The ultrastructure of the tissue was examined by TEM. Glucose and lactate analysis indicated that all groups had similar metabolic activity. TUNEL stain showed no significant difference in apoptotic damage induced by electrical stimulation compared to the control. Samples stimulated at 2 Hz exhibited reduced collagen deposition compared to the control and 1 Hz stimulated samples. Muscle‐protein marker desmin was highly expressed in constructs stimulated with 1 Hz/5 V sample. TEM revealed that the stimulated samples developed highly organized sarcomeres, which coincided with improved contractile properties in the 1 Hz/5 V‐ and 2 Hz/5 V‐stimulated groups. Our data implicate that a specific electrical frequency may modulate type I collagen accumulation and a specific voltage may affect the differentiation of muscle sarcomeres in excitable cells. Copyright © 2008 John Wiley & Sons, Ltd.Keywords
This publication has 35 references indexed in Scilit:
- Proteome Dynamics during C2C12 Myoblast DifferentiationMolecular & Cellular Proteomics, 2005
- Mechanical Properties and Remodeling of Hybrid Cardiac Constructs Made from Heart Cells, Fibrin, and Biodegradable, Elastomeric Knitted FabricTissue Engineering, 2005
- Engineering skeletal myoblasts: roles of three-dimensional culture and electrical stimulationAmerican Journal of Physiology-Heart and Circulatory Physiology, 2005
- Desmin Filaments Influence Myofilament Spacing and Lateral Compliance of Slow Skeletal Muscle FibersBiophysical Journal, 2005
- Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineeringNature Biotechnology, 2005
- Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffoldsProceedings of the National Academy of Sciences of the United States of America, 2004
- Establishment of a Simple and Practical Procedure Applicable to Therapeutic AngiogenesisCirculation, 1999
- What does chronic electrical stimulation teach us about muscle plasticity?Muscle & Nerve, 1999
- Early changes in performance, blood flow and capillary fine structure in rat fast muscles induced by electrical stimulationJournal Of Physiology-London, 1999
- Desmin in Muscle Formation and Maintenance: Knockouts and Consequences.Cell Structure and Function, 1997